🔅 Nucleic Acid Amplification Technics

– Some alternative nucleic acid amplification techniques are:

• Transcription mediated amplification (TMA): which uses RNA polymerase and reverse transcriptase to amplify RNA targets in an isothermal reaction.

• Loop-mediated isothermal amplification (LAMP): which uses a set of four or six primers and a strand-displacing DNA polymerase to amplify DNA targets in a single temperature.

• Helicase-dependent amplification (HDA): which uses a helicase enzyme and a DNA polymerase to separate and amplify DNA targets in an isothermal reaction.

• Clustered regularly interspaced short palindromic repeats (CRISPR): which uses a guide RNA and a Cas enzyme to target and cleave specific DNA sequences, generating detectable products.

• Strand displacement amplification (SDA): which uses a nicking endonuclease and a DNA polymerase to initiate and propagate the amplification of DNA targets in an isothermal reaction.

These techniques have different advantages and disadvantages over PCR, such as: speed, simplicity, sensitivity, specificity, cost, and equipment requirements.